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Journal: Journal of immunological methods
Article Title: Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies.
doi: 10.1016/j.jim.2021.113173
Figure Lengend Snippet: Fig. 2. Characterization of anti-mTNFR2 mAbs targeting CRDs 1–4. (A) Schematic representation of the 6 mouse-human TNF2 chimeras CRD1-CRD4 (Cystein Rich Domain). (B) The targeting CRD of each mAb were determined by cell ELISA with mouse-human TNFR2 domain swap mutants. Data represented as a three-parameter OD450–620 detection based on mean and SD of three independent experiments. (C) The domain epitopes of the 13 mAbs are indicated on a hTNFR2-hTNFα trimer structure (PDB: 3ALQ), 74% similar to mouse TNFR2. The CRDs for one TNFR2 receptor are shown in indicated colors.
Article Snippet: Immunoreactivity to mouse TNFR2 and cross-reactivity to human TNFR2 was assessed by ELISA using recombinant mTNFR2/Fc-protein (R&D Systems, 9707-R2) and
Techniques: Enzyme-linked Immunosorbent Assay