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R&D Systems htnfr2
Fig. 2. Characterization of anti-mTNFR2 mAbs targeting CRDs 1–4. (A) Schematic representation of the 6 mouse-human TNF2 chimeras CRD1-CRD4 (Cystein Rich Domain). (B) The targeting CRD of each mAb were determined by cell ELISA with mouse-human TNFR2 domain swap mutants. Data represented as a three-parameter OD450–620 detection based on mean and SD of three independent experiments. (C) The domain epitopes of the 13 mAbs are indicated on a <t>hTNFR2-hTNFα</t> trimer structure (PDB: 3ALQ), 74% similar to mouse TNFR2. The CRDs for one TNFR2 receptor are shown in indicated colors.
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Fig. 2. Characterization of anti-mTNFR2 mAbs targeting CRDs 1–4. (A) Schematic representation of the 6 mouse-human TNF2 chimeras CRD1-CRD4 (Cystein Rich Domain). (B) The targeting CRD of each mAb were determined by cell ELISA with mouse-human TNFR2 domain swap mutants. Data represented as a three-parameter OD450–620 detection based on mean and SD of three independent experiments. (C) The domain epitopes of the 13 mAbs are indicated on a hTNFR2-hTNFα trimer structure (PDB: 3ALQ), 74% similar to mouse TNFR2. The CRDs for one TNFR2 receptor are shown in indicated colors.

Journal: Journal of immunological methods

Article Title: Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies.

doi: 10.1016/j.jim.2021.113173

Figure Lengend Snippet: Fig. 2. Characterization of anti-mTNFR2 mAbs targeting CRDs 1–4. (A) Schematic representation of the 6 mouse-human TNF2 chimeras CRD1-CRD4 (Cystein Rich Domain). (B) The targeting CRD of each mAb were determined by cell ELISA with mouse-human TNFR2 domain swap mutants. Data represented as a three-parameter OD450–620 detection based on mean and SD of three independent experiments. (C) The domain epitopes of the 13 mAbs are indicated on a hTNFR2-hTNFα trimer structure (PDB: 3ALQ), 74% similar to mouse TNFR2. The CRDs for one TNFR2 receptor are shown in indicated colors.

Article Snippet: Immunoreactivity to mouse TNFR2 and cross-reactivity to human TNFR2 was assessed by ELISA using recombinant mTNFR2/Fc-protein (R&D Systems, 9707-R2) and hTNFR2 (R&D Systems, 726-R2) as well as CHO-K1.mTNFR2 and CHO-K1.hTNFR2.

Techniques: Enzyme-linked Immunosorbent Assay